之前一直用的是muscle,看到一个文章使用了Kalign,尝试一下吧
安装
wget -c https://github.com/TimoLassmann/kalign/archive/refs/tags/v3.4.0.tar.gz
tar -zxvf v3.4.0.tar.gz
cd kalign-3.4.0
mkdir build
cd build
cmake ..
make
make test
sudo make install
用法
Usage: kalign -i <seq file> -o <out aln>--type: Alignment type (rna, dna, internal). [rna]Options: protein, divergent (protein) rna, dna, internal (nuc).
-n/--nthreads:Number of threads. [4]